Plasmid_Backbone
Part:BBa_K4298008
Designed by: Buyao Li Group: iGEM22_Peking (2022-09-29)
AcrB empV
Backbone of AcrB plasmid, acting as the negative control.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 3104
Illegal SpeI site found at 908 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SpeI site found at 908
Illegal NotI site found at 915 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BamHI site found at 2108
Illegal XhoI site found at 2143
Illegal XhoI site found at 4066 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 3104
Illegal SpeI site found at 908 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 3104
Illegal SpeI site found at 908
Illegal NgoMIV site found at 2259
Illegal AgeI site found at 2672 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 4141
[edit]
Categories
Parameters
//chassis/prokaryote/bcepacia
//plasmidbackbone/copynumber/inducible
//plasmidbackbone/copynumber/inducible
None |